Prevalencia del determinante de resistencia plasmídica a quinolonas aac(6’)-Ib-cren cepas de Escherichia coli y Klebsiella pneumoniae productoras de BLEE aisladas en diez hospitales de Chile / Prevalence of plasmid-mediated quinolone resistance determinant aac(6’)-Ib-cr among ESBL producing enterobacteria isolates from Chile an hospitals

Introducción: En Chile no se conoce la frecuencia del determinante plasmídico de resistencia a quinolonas(DPRQ) aac(6 )-Ib-cr en cepas de Klebsiella pneumoniae y Escherichia coli productoras de BLEE. Metodología: Utilizando PCR, RFLP y secuenciación se detectó aac(6 )-Ib y aac(6 )-Ib-cr en cepas aisladas en 10 hospitales chilenos entre 2008 y 2009.Resultados: Este DPRQ fue detectado ampliamente en K. pneumoniae (54%) y E. coli (74%). La CIM50de CIP fue mayor en cepas con aac(6’)-Ib-cr; 8 veces en K. pneumoniae y 4 en E. coli. En 13 cepas deK. pneumoniae y 3 de E. coli se encontraron ambos genes simultáneamente. Conclusión: Este es el primer informe de aac(6’)-Ib-cr en cepas de K. pneumoniae y E. coli productoras de BLEE aisladas en hospitales chilenos distribuidos en una extensión geográflca superior a 2.800 km (AU)

Introduction: The frequency of aac(6 )-Ib-cr gene in ESBL-producing strains of Klebsiella pneumoniae and Escherichia coli is unknown, in Chile. Methodology: The aac(6 )-Ib and aac(6’)-Ib-cr genes were investigated using polymerase chain reaction(PCR), restriction fragment length polymorphism (RFLP), and sequencing, in strains isolated from 10Chilean hospitals between 2008-2009.Results: The aac(6’)-Ib-cr gene was detected in 54% of K. pneumoniae and 74% of E. coli strains. The CIM50 of CIP was higher among strains harboring aac(6’)-Ib-cr, 8 times higher in K. pneumoniae and 4 times higherin E. coli. Moreover, both aac(6’)-Ib and aac(6’)-Ib-cr were simultaneously found in 13 K. pneumoniae and3 E. coli isolates. Conclusion: This is the first report of aac(6’)-Ib-cr in ESBL-producing strains of K. pneumoniae and E. coliisolated from in-patients in Chilean hospitals located along an area of more than 2,800 Km (AU)

[Prevalence of plasmid-mediated quinolone resistance determinant aac(6')-Ib-cr among ESBL producing enterobacteria isolates from Chilean hospitals]. / Prevalencia del determinante de resistencia plasmídica a quinolonas aac(6')-Ib-cr en cepas de Escherichia coli y Klebsiella pneumoniae productoras de BLEE aisladas en diez hospitales de Chile.

INTRODUCTION: The frequency of aac(6')-Ib-cr gene in ESBL-producing strains of Klebsiella pneumoniae and Escherichia coli is unknown, in Chile. METHODOLOGY: The aac(6')-Ib and aac(6')-Ib-cr genes were investigated using polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and sequencing, in strains isolated from 10 Chilean hospitals between 2008-2009. RESULTS: The aac(6')-Ib-cr gene was detected in 54% of K. pneumoniae and 74% of E. coli strains. The CIM(50) of CIP was higher among strains harboring aac(6')-Ib-cr, 8 times higher in K. pneumoniae and 4 times higher in E. coli. Moreover, both aac(6')-Ib and aac(6')-Ib-cr were simultaneously found in 13 K. pneumoniae and 3 E. coli isolates. CONCLUSION: This is the first report of aac(6')-Ib-cr in ESBL-producing strains of K. pneumoniae and E. coli isolated from in-patients in Chilean hospitals located along an area of more than 2,800 Km.